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Serologic Cross-Reactivity Between H-2 and HL-A Antigens

I. Specific Reactivity of Rabbit anti-HL-A Sera against Murine Cells¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Soluble HL-A antigen extracted from cultured human lymphoid cells (RPMI 1788, HL-A 2, 10, 7, W14) by the 3M KCl method was injected into rabbits. The rabbit anti-HL-A sera contained antibodies against all the HL-A specificities present on the immunogen. Moreover, these same xenoantibodies reacted also with certain murine lymphocytes bearing distinct H-2 specificities in the direct complement dependent lymphocytotoxic test. Absorption studies confirmed this selected reactivity and led to the conclusion that mainly antigenic determinants of the D region of H-2^d and the K region of H-2^b are involved in this cross-reactivity. Rabbit anti-HL-A sera absorbed with cells of different mouse strains were tested against a large panel of human lymphocytes from unrelated donors for remaining cytotoxic activity. The results indicate that absorption with cells of the genotypes H-2^b (K^bD^b) and H-2^a (K^kD^d) remove antibodies against determinants of the first segregant series (cross-reacting with HL-A 2 and 10).

These data suggest a specific cross-reactivity between human and murine histocompatibility antigens and suggest a certain structural homology between these cell surface antigens and perhaps even a similar organization of the genetic fine structure of the HL-A and H-2 systems.

Footnotes

¹ This is publication 576 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. This work was supported by United States Public Health Service Grants AI-10180 and CA-10596 from the National Institutes of Health and Grant 70-615 from the American Heart Association, Inc.

² D. G. is supported by the Deutsche Forschungsgemeinschaft, SFB 37 (University Munich), Germany.

³ S. F. is a visiting scientist from the University of Milan, Italy.