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The Journal of Immunology, 1972, 109: 371-381.
Copyright © 1972 by The American Association of Immunologists, Inc.
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The Carbohydrate Composition of Immunoglobulins from Diverse Species of Vertebrates1

R. T. Action2, W. Niedermeier3, P. F. Weinheimer4, L. W. Clem, G. A. Leslie and J. C. Bennett5

From the Division of Biology, California Institute of Technology, Pasadena, California 91109; Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama in Birmingham, Alabama 35233; Department of Microbiology, College of Medicine, University of Florida, Gainesville, Florida 32601; Department of Microbiology and Immunology, Tulane University School of Medicine, New Orleans, Louisiana 70112

Abstract

Immunoglobulins and their respective heavy (H) and light (L) polypeptide chains from species representing the major classes of vertebrates were analyzed for their carbohydrate composition by gas chromatography of the alditol acetate derivatives of the monosaccharides released by acid hydrolysis. Mannose, galactose, glucosamine and sialic acid were present in the immunoglobulins from all the species investigated. Fucose however was absent from the immunoglobulins of two species of animals studied. Most of the carbohydrate was found associated with the H chains. The carbohydrate composition was felt to be heterogeneous in that the quantities of fucose and sialic acid found often accounted for less than one residue per H chain. Whereas species representative of the mammals and birds had mannose to galactose ratios greater than one, the ratio of these sugars to each other in the immunoglobulins from animals below the birds was on the order of one.

Footnotes

1 This study was supported by grants from the United States Public Health Service (AI-02693, AI-9153, AM-9153, AM-03555 and GRS-5501-FR-05377-09), the National Science Foundation (GB-5983, GB-31-1 and GB-8632), the Atomic Energy Commission (Contract AT(04-3)-767) and the John A. Hartford Foundation.

2 Smith Kline and French Research Fellow, California Institute of Technology.

3 Address reprint requests to Dr. W. Niedermeier at the University of Alabama in Birmingham.

4 Postdoctoral Research Fellow supported by United States Public Health Service Fellowship FO2AM42874-01A1.

5 Recipient of a Research Career Development Award (K3GM-25,386) and a Markle Scholar in Academic Medicine.






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