HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Niederhuber, J. E. Articles by Berquist, R. Search for Related Content PubMed Articles by Niederhuber, J. E. Articles by Berquist, R.

A Specific Mouse B Lymphocyte Antigen (MBLA) Demonstrated by Double Immunofluorescence¹

From the Division of Immunobiology, Karolinska Institute Wallenberglaboratory, Lilla Freskati, 10405 Stockholm 50, Sweden and Division of Immunology, School of Medicine, Karolinska Institute, Stockholm 60, Sweden

Abstract

Double immunofluorescent staining was used to demonstrate the presence of a specific antigenic marker (MBLA) on the surface of B lymphocytes in the mouse. The percentages of thymus dependent and thymus independent cells detected by indirect staining of specific antisera-treated cells were consistent with the cytotoxic indices for these sera. The double staining of anti-MBLA-treated cells after incubation with fluorescein-conjugated anti-mouse immunoglobulin serum and rhodamine-conjugated anti-rabbit immunoglobulin serum confirmed the absence of anti-immunoglobulin activity in the anti-MBLA serum.

Footnotes

¹ This work was supported by the Swedish Medical Research Council, the Swedish Cancer Society, the Jeanssan Foundation, Anders Otto Swäds Stiftelse, and the Wallenberg Foundation.

² Visiting fellow; permanent address: Section of General Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48104.

³ Present address: Department of Microbiology, School of Medicine, State University of New York at Buffalo, Buffalo, New York 14214.