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The Journal of Immunology, 1972, 109: 353-359.
Copyright © 1972 by The American Association of Immunologists, Inc.
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Reaction Mechanisms of Nascent C567 (Reactive Lysis)

I. Reaction Characteristics for Production of EC567 and Lysis by C8 and C91

John N. Goldman2, Shaun Ruddy3 and K. Frank Austen

From the Department of Medicine, Harvard Medical School at the Robert B. Brigham Hospital, Boston, Massachusetts 02120

Abstract

An activated complex of the fifth and sixth components of complement (C
Figure 1
) was generated by treatment of certain human sera with either zymosan or the low molecular weight anticomplementary factor of cobra venom. The interaction of C
Figure 2
with C7 and unsensitized erythrocytes was studied. The formation of EC
Figure 3
from these reactants was temperature dependent and proceeded most favorably at pH 6.0, ionicity 0.065. The half-life of the hemolytic activity of nascent C
Figure 4
was 5 hr at 0°C and less than 2 min at 30°C. When the average numbers of C
Figure 5
sites per erythrocyte generated in the presence of optimal C7 were plotted as a function of C
Figure 6
input, linear responses were obtained, indicating that a single competent C
Figure 7
site is sufficient to prepare the cell for lysis by C8 and C9. Nascent
Figure 8
formed by the interaction of C
Figure 9
and C7 operates in the absence of antibody and extends the membrane-damaging capacity of the complement system to unsensitized cells.

Footnotes

1 This work was supported by Grants AI-07722 and RR-05669 from the National Institutes of Health and grants from the Massachusetts Arthritis Foundation and the John A. Hartford Foundation, Inc.

2 Recipient of a Special Postdoctoral Research Fellowship AI-52, 051-01 from the National Institutes of Health.

3 Investigator of the Howard Hughes Medical Institute.






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