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Secretory Newcastle Disease Virus Antibodies from Chicken Respiratory Tract

From the Department of Virology, The Hebrew University-Hadassah Medical School, Jerusalem, Israel

Abstract

The nature of the antibodies extracted from lungs of chickens immunized intranasally by Newcastle disease virus (NDV) was analyzed by chromatography on Sephadex G-200, DEAE cellulose, as well as by electrophoresis on acrylamide gel. Specific local antibody activity was always located at the same region as serum antibodies with a molecular weight of 160,000 to 180,000. By immunodiffusion and immunoelectrophoresis this antibody was shown to be antigenically identical to humoral IgG serum antibodies. Concentrations of hexosamine were 1.55% for serum and 1.35% for lung antibodies. The S values as determined by analytical ultracentrifugation were 6.9 and 7.1 for local and humoral antibodies, respectively. It was concluded, therefore, that local anti-NDV antibodies belong to the IgG type of immunoglobulin, and that this immunoglobulin is responsible for the systemic as well as for the local immunity in the chickens.