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Studies in Experimental Eosinophilia

IX. Inhibition by Puromycin of the Eosinophil Response Which Hemocyanin Elicits in Guinea Pig Lymph Nodes¹

From the Channing Laboratory, Boston City Hospital and the Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts

Abstract

Puromycin blocked the eosinophil response which is elicited in guinea pig lymph nodes by a single injection of hemocyanin into footpads. The drug was most effective in interfering with the 24-hr response when it was given just before or no later than 4 hr after the antigen. A partial block was noted when the drug preceded the antigen by 2 to 8 hr. Interference with the 15-min vascular response required a higher dose. The secondary type of eosinophil response, which is seen in an immunized animal, was not inhibited by puromycin. The drug was effective when administered into the footpads, but a higher dose was required when it was given by the intraperitoneal route. The results support the notion that the eosinophilia which occurs during the first few minutes of a primary immune response reflects the de novo synthesis of antibody, rather than the presence of preformed antibody.

Footnotes

¹ This work was supported in part by United States Public Health Service Grant AI-04716 and by a grant-in-aid from the American Heart Association.