HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bacon, L. D. Articles by McBride, R. A. Search for Related Content PubMed Articles by Bacon, L. D. Articles by McBride, R. A.

Alloimmune Hemolytic Plaque-Forming Cells: Kinetics of IgM and IgG Alloantibody Releasing Cells during a Primary and Secondary Immune Response of Chickens¹

From the Department of Pathology, New York Medical College, New York, New York 10029

Abstract

The kinetics of alloimmune hemolytic plaque-forming cells found in spleens of chickens after primary and secondary immunization was investigated. Rabbit anti-chicken immunoglobulin was employed for plaque development since chicken alloantibodies are non-hemolytic. Plaques formed by cells secreting IgM and IgG were developed separately by using rabbit antisera specific for each class of immunoglobulin.

The kinetics as well as numbers of cells which produced antibodies specific for B blood group alloantigens during both the primary and secondary response were similar to what has been found for the mouse heteroimmune system. Certain advantages of the chicken alloimmune system for enumeration of hemolytic plaque-forming cells are discussed.

Footnotes

¹ Supported by Grant AM-13268 from the United States Public Health Service.

² Recipient of United States Public Health Service Post-doctoral Fellowship 1 FO2 AM44240. Present address: The Center for Immunology, State University of New York at Buffalo, Buffalo, New York 14214.

³ Recipient of Career Scientist Award of the Health Research Council of The City of New York: Contract I-591.

⁴ Please send reprint requests to Raymond A. McBride.

This article has been cited by other articles:

				B. Ask, E. H. van der Waaij, E. J. Glass, and S. C. Bishop Modeling Immunocompetence Development and Immunoresponsiveness to Challenge in Chicks Poult. Sci., July 1, 2007; 86(7): 1336 - 1350. [Abstract] [Full Text] [PDF]

				E Le Rouzic, P Thoraval, M Afanassieff, Y Cherel, G Dambrine, and B Perbal Alterations of the MDV oncogenic regions in an MDV transformed lymphoblastoid cell line Mol. Pathol., August 1, 2002; 55(4): 262 - 272. [Abstract] [Full Text] [PDF]