HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Butler, J. E. Articles by Kiddy, C. A. Search for Related Content PubMed Articles by Butler, J. E. Articles by Kiddy, C. A.

Studies on the Relative Synthesis and Distribution of IgA and IgG1 in Various Tissues and Body Fluids of the Cow

From the United States Department of Agriculture and the National Institutes of Health, Bethesda, Maryland 20014

Abstract

In vitro synthesis of bovine IgA was detected by a radioimmunoelectrophoretic assay in 17 of 20 different tissues obtained from cows and 2 of 6 different tissues obtained from a 1-week-old calf. Conclusions drawn from these qualitative studies are supported by the results of preliminary quantitative data. Synthesis of IgA was most pronounced in the adult ileum, duodenum, colon, lungs, lower nasal mucosa, oral pharyngeal mucosa, parotid gland, lacrimal gland and thymus and the calf parotid and lacrimal glands. Correspondingly, IgA, not IgG1, is the principal immunoglobulin in bovine tears and saliva. In comparison to other secretory tissues of the cow and the mammary glands of the rabbit, human and monkey, the bovine mammary gland shows moderate deficiency in the synthesis of IgA. This deficiency is reflected in the fact that IgA does not become the predominant immunoglobulin of normal bovine milk, although an increase in the IgA to IgG1 ratio does occur after parturition. The adult bovine thymus is an immunologically active secretory tissue, but the ileum of the 1-week-old calf appears to lack an IgA-producing system. The concentration of serum IgA is lower than in the human and is greater before calving than after. Most of the IgG1 synthesized in the mammary gland is eluted from Sephadex G-200 before 7S IgG1.

Footnotes

¹ This work was performed at Dairy Products Laboratory, Eastern Marketing and Nutrition Research Division, Agricultural Research Service, United States Department of Agriculture, Washington, D. C. 20250.

² Present address: Department of Microbiology, College of Medicine, University of Iowa, Iowa City, Iowa 52240.

³ National Institutes of Health, Bethesda, Maryland 20014.

⁴ Animal Science Research Division, Agricultural Research Division, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705.

This article has been cited by other articles:

				H. M. Farrell Jr., R. Jimenez-Flores, G. T. Bleck, E. M. Brown, J. E. Butler, L. K. Creamer, C. L. Hicks, C. M. Hollar, K. F. Ng-Kwai-Hang, and H. E. Swaisgood Nomenclature of the Proteins of Cows' Milk--Sixth Revision J Dairy Sci, June 1, 2004; 87(6): 1641 - 1674. [Abstract] [Full Text] [PDF]

				J. E. Butler, J. Sun, P. Weber, S. P. Ford, Z. Rehakova, J. Sinkora, and K. Lager Antibody Repertoire Development in Fetal And Neonatal Piglets. IV. Switch Recombination, Primarily in Fetal Thymus, Occurs Independent of Environmental Antigen and Is Only Weakly Associated with Repertoire Diversification J. Immunol., September 15, 2001; 167(6): 3239 - 3249. [Abstract] [Full Text] [PDF]

				J. E. Butler, P. Weber, M. Sinkora, J. Sun, S. J. Ford, and R. K. Christenson Antibody Repertoire Development in Fetal and Neonatal Piglets. II. Characterization of Heavy Chain Complementarity-Determining Region 3 Diversity in the Developing Fetus J. Immunol., December 15, 2000; 165(12): 6999 - 7010. [Abstract] [Full Text] [PDF]