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Ascaris Cuticle Collagen and Mammalian Collagens: Cell Mediated and Humoral Immunity Relationships¹

From the Departments of Biochemistry and Surgery, University of California, San Francisco, California 94122, and Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot, Israel

Abstract

Complete cell-mediated and humoral cross-reactivity was demonstrated between native Ascaris cuticle collagen and its reduced and carboxymethylated (RCM) form. The data indicate the preservation of intact immunologic determinants after reduction and alkylation of the parent molecule.

Cell-mediated immunity could be demonstrated with both forms of Ascaris collagen and human collagen whereas no such cross-reaction existed between the former collagens and mouse collagen. No humoral cross-reactivity between Ascaris collagen and a variety of mammalian collagens was detectable.

The data demonstrate the usefulness of cell-mediated immunity in determining the relatedness of phylogenetically remote proteins. The possibilities that Ascaris and mammalian collagens may be products of either divergence from a common primordial protein or convergence from different primordial proteins are discussed.

Footnotes

¹ This study was supported in part by United States Public Health Service Grants GM-18470 and HD-05894. Dr. Michaeli is a recipient of Research Career Development Award 5-K4-AM-50, 205-01 from the National Institutes of Health. Reprint requests may be addressed to Dr. Michaeli, Department of Surgery, University of California School of Medicine, San Francisco, California 94122.

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