HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Waldo, E. D. Articles by Zucker-Franklin, D. Search for Related Content PubMed Articles by Waldo, E. D. Articles by Zucker-Franklin, D.

Ultrastructure and Immunofluorescence of Mouse Spleen Cells Obtained by Discontinuous Albumin Gradient Centrifugation

From the Department of Pathology and Department of Medicine, New York University Medical Center, New York, New York 10016

Abstract

The ultrastructural, fluorescent and phagocytic properties of mouse spleen cells separated by differential centrifugation in discontinuous albumin gradients were studied. Four bands were consistently obtained. The most dense, and most homogeneous band (D) consisted chiefly of small lymphocytes. Large lymphocytes, monocytes and cells undergoing mitoses occurred chiefly in the least dense layer. All fractions contained about similar numbers of plasma cells. Approximately 50% of the cells in band D had surface immunoglobulin whereas only very few cells in the less dense layers showed surface immunofluorescence. The observations should prove useful for the interpretation of data obtained from experiments in which cells from albumin gradients have been employed.

Footnotes

¹ This work was supported by United States Public Health Service Grant AM 12274 and United States Public Health Service Research Career Development Award 5 K03 AI 09572.