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Interaction in Vivo of Homocytotropic Antibodies Belonging to Two Different Rat Immunoglobulin Classes: Effect of IgE on the Passive Cutaneous Anaphylactic Reaction Mediated by IgG_a Antibodies¹

From the Allergy, Arthritis and Clinical Immunology Units of the Medical Services, Massachusetts General Hospital and the Department of Medicine, Harvard Medical School, Boston, Massachusetts

Abstract

Rat anti-Nippostrongylus brasiliensis antiserum was considerably more effective than normal rat serum in inhibiting the passive cutaneous anaphylactic (PCA) reaction mediated by an IgG_a anti-dinitrophenyl (DNP) antiserum fraction. The inhibitory activity had the chromatographic, gel filtration and heat lability characteristics of rat IgE. The IgG_a fraction, isolated from hyperimmune rat anti-DNP antiserum, devoid of IgE anti-DNP activity, yielded stronger 6-hr PCA reactions than did whole antiserum. The inhibitory activity in whole antiserum was attributed to nonspecific IgE, although the existence of other, minor, inhibitors could not be excluded. Rats repeatedly infected with N. brasiliensis larvae failed to undergo PCA reactions at sites prepared with certain IgG_a or IgE antibody-containing fractions; it is presumed that in these animals mast cell receptors are occupied by autologous IgE anti-worm antibodies. These experiments provide in vivo confirmation of the interaction of rat IgE and IgG_a antibodies at a common receptor on target cells.

Footnotes

¹ This is publication No. 556 of the Robert W. Lovett Memorial Group for the Study of Diseases Causing Deformities, Massachusetts General Hospital, Boston, Massachusetts. This study was supported by Grants AI-02638, AI-10129, AM-3564 and AM-5067 from the National Institutes of Health, and a grant from the Arthritis Foundation, Massachusetts Chapter.