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The Journal of Immunology, 1972, 108: 1624-1630.
Copyright © 1972 by The American Association of Immunologists, Inc.
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The Functional Capacity of X-Irradiated Macrophages1

Jon R. Schmidtke2 and Frank J. Dixon

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037

Abstract

Graded doses of x-irradiation (up to 10,000 R) had no effect on the uptake and little effect on the catabolism of keyhole limpet hemocyanin (KLH) or human {gamma}-globulin (HGG) in vitro by macrophages from non-immunized CBA or A mice. After uptake and catabolism, however, greater amounts of KLH and HGG remained associated with the plasma membrane of x-irradiated (10,000 R) macrophages compared with control cells. Because of their greater surface binding of antigens, x-irradiated macrophages after transfer to normal mice were more effective in inducing immune responses to antigens to which they were exposed in vitro than were normal macrophages. This increased immunogenicity of KLH bound to x-irradiated macrophages was abrogated by treating the cells with trypsin before transfer. In addition, transfer of normal macrophages carrying KLH or HGG to sublethally irradiated mice did not reconstitute their ability to form antibody to these proteins.

Footnotes

1 This is publication 567 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. This research was supported by Atomic Energy Commission Contract AT(04-3)-410 and United States Public Health Service Grant AI-07007.

2 Supported by Training Grant 5T1GM683 from the United States Public Health Service.






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