HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wyatt, H. V. Articles by Borsos, T. Search for Related Content PubMed Articles by Wyatt, H. V. Articles by Borsos, T.

Production of the Second (C2) and Fourth (C4) Components of Guinea Pig Complement by Single Peritoneal Cells: Evidence That One Cell May Produce Both Components¹

From the Biology Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Guinea pig peritoneal cells were examined by a hemolytic plaque assay for their ability to produce C4 and C2. Of cells obtained by peritoneal wash or from exudates induced by starch, 12% were capable of producing C2 and 12% were capable of producing C4. Peritoneal cells induced by oil or casein showed fewer cells capable of producing C4 or C2. With a plaque assay specifically designed to detect only those peritoneal cells that were producing both C4 and C2, we found one hemolytic plaque (produced by a single cell) per 1000 peritoneal cells. Cells capable of producing both C4 and C2 were identified as large mononuclear cells. The production of C4 and C2 by these cells was a temperature dependent process.

Footnotes

¹ Presented in part at the national meeting of the Federation of American Societies for Experimental Biology, April 1971, Chicago, Ill.

² Visiting Scientist, from the School of Applied Biology, University of Bradford, United Kingdom.

³ Present address: Harvard Medical School, Children's Hospital Medical Center, Boston, Mass. 02115.

⁴ Reprint requests to: Dr. Borsos, National Cancer Institute, Building 37, Room 2B15, Bethesda, Md. 20014.