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Studies of Non-Migrating, Long-Lived Lymphocytes in Rats

IV. Comparison of Density Distributions with Flagellin-Binding Cells and Antibody-Forming Cell Precursors¹

From the Children's Hospital at Stanford, Department of Pediatrics, Stanford University School of Medicine, Stanford, California 94305, and the Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, 3050, Australia

Abstract

Rat lymph node lymphocytes were labeled by hind footpad injections of tritiated thymidine in the absence of an immune stimulus or after priming with a polymerized Salmonella flagellin. Fifty or more days later the rats were killed and the lymph node cells were fractionated on continuous albumin density gradients. The proportions of lymphocytes of lighter density (< 1.07 g/cm³) were greater in primed than in unprimed nodes. The density distributions of the "long-lived" cells, i.e., those with persistent label, were identical to those of the total lymph node cell population. After challenge with either antigenically identical or different flagellins, the density distribution of the persistently labeled cells demonstrated relative loss of cells in the density range from 1.068 to 1.072 g/cm³ in comparison to the bulk of lymph node cells, but not in patterns distinct for the specificity of the antigens used for challenge. Comparison of the density distributions of long-lived lymphocytes with flagellinbinding cells and with cells transferring adoptive immune reactions revealed partial overlap with these cells from primed but not from unprimed lymph nodes.

Footnotes

¹ Supported in part by grants from the United States Public Health Service, AI-08490, and The John A. Hartford Foundation (J.J.M.), and from the National Health and Medical Research Council, Australia (K.S.).