HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hirata, A. A. Articles by Terasaki, P. I. Search for Related Content PubMed Articles by Hirata, A. A. Articles by Terasaki, P. I.

Masking of Human Transplantation Antigens by Diverse Substances¹

From the Department of Molecular Biology, Abbott Laboratories, North Chicago, Illinois 60064 and the Department of Surgery, School of Medicine, University of California, Los Angeles, California 90024

Abstract

The reaction of HL-A antibodies with the HL-A determinants on the surface of lymphocytes can be blocked by preincubation of lymphocytes with diverse substances such as normal human serum, autologous plasma, fetal calf serum, newborn serum, heat-inactivated rabbit serum and guinea pig serum, serum albumins from various species, lactalbumin, dextrans, synthetic polyglucose, and polyvinylpyrrolidone. The inhibitors can be washed off readily from lymphocytes. The inhibition procedure described here differs from the conventional inhibition tests in that the inhibitor is added first to the target cells. This "masking inhibition" procedure produces substantially greater inhibition than the standard inhibition methods in which the inhibitor is added first to antiserum before the addition of the target cells. The nonspecific nature of masking inhibition indicates that numerous substances adhere to the lymphocyte surface at HL-A sites or closely adjacent sites.

Footnotes

¹ This work was supported in part by Research Grants AI 04444, AM 02375, and AM 7513 from the National Institutes of Health, United States Public Health Service.