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The Production of Anti-Dnp Antibody in the Bullfrog, Rana Catesbiana¹

From the Departments of Medical Microbiology and Medicine, Stanford University School of Medicine, Stanford, California 94305

Abstract

Bullfrogs injected with dinitrophenyl keyhole limpet hemocyanin (DNP-KLH) in complete Freund's adjuvant produced precipitating anti-DNP antibody with characteristics different from that produced by frogs immunized against DNP-Salmonella in saline.

The antibody from animals injected with DNP-KLH sedimented as a 7S protein on sucrose density gradients and gave precipitin curves similar to the precipitin curve of mammalian 7S-IgG except for the lack of 100% precipitability of the antigen over most of the frog curve.

DNP-Salmonella induced antibody which sedimented as a macroglobulin and was distinguished from the light antibody induced by DNP-KLH by radioimmunoelectrophoresis. The precipitin curve for this immunoglobulin and the persistence of high titers 1 year after a single injection suggest that the immunoglobulin is IgM.

Footnotes

¹ This work was supported by National Science Foundation Grant GB-15748; American Cancer Society, California Division, Grant 450; American Cancer Society Institutional Grant 2 PGD 602; and Stanford University General Research Support Grant Suballocation 5 SO 1 RR 05353-10.