The JI
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     
 

The Journal of Immunology, 1972, 108: 1431-1438.
Copyright © 1972 by The American Association of Immunologists, Inc.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Takeda, Y.
Right arrow Articles by Pressman, D.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Takeda, Y.
Right arrow Articles by Pressman, D.

Peptides from the Site of Rabbit Antibody: Anti-Azophenyltrimethylammonium Antibody1

Yasushi Takeda2, Bernard Friedenson, Oliver A. Roholt and David Pressman

From the Department of Biochemistry Research, Roswell Park Memorial Institute,3 Buffalo, New York 14203

Abstract

A tyrosine peptide which appears to be derived from the combining site of an anti-azophenyltrimethylammonium antibody was isolated and the amino acid sequence was determined. Binding studies showed that the antibody was inactivated by iodination and that the attack was in the site, since the presence of the hapten, phenyltrimethylammonium ion, during iodination prevented the loss of sites.

The peptide from the site was isolated after a paired labeling procedure as follows. One portion of the antibody was iodinated with 125I-labeled hypoiodite. A second portion was iodinated with 131I-labeled hypoiodite in the presence of hapten to protect the site from iodination. The protected and unprotected preparations were mixed, digested with pepsin and the iodinated peptides separated. The ratio of 125I:131I relative to that of the unfractionated digest was determined for the peptides; a high ratio indicated that the peptide was derived from the antibody site. A high-ratio peptide, equivalent to at least 37% of the total antibody sites present, had the sequence Ile-DIT-Asp. The peptide was shown to be derived from the heavy chain of the antibody and was probably from the variable portion.

Footnotes

1 This work was supported in part by Grant AI-3962 from the National Institute of Allergy and Infectious Diseases.

2 On leave of absence from the Faculty of Pharmaceutical Sciences, University of Tokyo, Bunkyo-ku, Tokyo, Japan.

3 A unit of the New York State Department of Health.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Website Copyright © 1972 by The American Association of Immunologists, Inc. All rights reserved.
All Contents Copyright © 1972 by The American Association of Immunologists, Inc. All rights reserved.