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Serologic Specificities of Brain Subcellular Organelles

I. Antisera to Synaptosomal Fractions¹

From the Laboratory of Nuclear Medicine and Radiation Biology, and the Department of Biological Chemistry, University of California School of Medicine at Los Angeles, Los Angeles, California and the Department of Psychobiology, University of California, Irvine, California

Abstract

Antisera were prepared to synaptosomal fractions isolated from rat cerebral cortex. The antisera, at optimal dilutions, reacted in the complement fixation reaction only with the synaptosomal fraction and synaptosomal plasma membrane fractions, but not with myelin, nuclei, soluble protein, mitochondria or synaptic vesicles from rat cortex. Little or no reaction was observed with membrane preparations from other rat organs. The specific antigenic determinants of the synaptosomal membrane were relatively stable to heat, sonication and mild tryptic digestion. These results demonstrate that the plasma membrane of cortical synaptosomes can be serologically distinguished from the plasma membrane of other organs and from the rest of the subcellular and sub-synaptic fractions of brain.

Footnotes

¹ This work was supported by Contract AT(04-1) GEN-12 between the Atomic Energy Commission and the University of California, and Grant N. B. 08597 from the National Institutes of Health.