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Induction of Antiidiotypic Antibodies Directed to Antibenzoate Antibody in Rabbits Actively Synthesizing High Titers of Antibenzoate Antibody¹

From the Departments of Biological Chemistry and Surgery, University of Illinois at the Medical Center, Chicago, Illinois 60680

Abstract

In efforts to elicit antiidiotypic antibodies, each of 16 rabbits was immunized with purified antibenzoate antibodies prepared in an individual donor rabbit of allotype matched to the recipient. Two types of recipients were used: 1) rabbits hyperimmune to the benzoate hapten; 2) rabbits hyperimmune to the noncross-reacting hapten, DNP. Nearly all recipient rabbits responded by producing anti-idiotypic antibodies, and no significant quantitative differences were detected in the two groups. This result implies that the rabbits producing antibenzoate antibodies at the time of immunization were not tolerant to the antibenzoate antibodies injected.

Efforts were also made to elicit antiidiotypic antibodies by self-immunization of donor rabbits with their own antibenzoate antibodies after rest periods totaling 23 to 36 weeks. Such experiments were unsuccessful, indicating that a state of tolerance to the rabbit's own antibodies had persisted.

Thus rabbits remained tolerant to their own idiotypic determinants, whereas the capacity to respond of other rabbits, actively synthesizing high titers of antibenzoate antibody, was not impaired. The results emphasize the broad spectrum of idiotypic specificities in different rabbits on molecules specific for a single hapten group.

Footnotes

¹ This work was supported by Grants from the National Institutes of Health (AI-06281 and AI-10220).

² Recipient of an Arthritis Foundation Postdoctoral Fellowship.

³ Supported by a National Institute of Medical Sciences Surgery Academic Training Grant (GM-1930).