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Persistence and Characterization of Idiotypes in Pedigreed Rabbits Producing Antibodies of Restricted Heterogeneity to Pneumococcal Polysaccharides

From the Laboratory of Immunology, National Institute of Allergy and Infectious Diseases and Laboratory of Microbiology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Anti-idiotype antisera were prepared which reacted with anti-SVIII antibodies of restricted heterogeneity in the sera of two rabbits, each from a different pedigree. No sharing of the idiotypes was detectable in antisera from close relatives of each donor. We looked for shared idiotypic determinants by precipitation tests and by an inhibition of binding assay which we estimate could have detected 1% of antibody with the same idiotype. Antibody to SVIII with idiotypic determinants present during the first course of immunization of an individual rabbit reappeared during the second immunization series 12 months later. During the course of restimulation, the antiserum exhibited increased complexity. By the 4th week, antibody with the original idiotype had decreased in concentration while the total anti-SVIII reached maximum concentration.

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