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Electron Microscopic Localization of Rabbit Immunoglobulin Allotype b4 on Blood Lymphocytes by an Indirect Ferritin Immune Complex Labeling Technique¹

From the Department of Pathology, University of California, San Diego, Medical School, La Jolla, California 92037

Abstract

Immunoglobulin allotypic determinant b4 may be detected on the surface of rabbit peripheral blood lymphocytes by electron microscopy. Soluble complexes of ferritin and rabbit antiferritin of a given allotype were incubated with lymphocytes coated with anti-allotype antibody to form labeled lymphocytes. Seventy to 80% of the lymphocytes examined demonstrated specific focal ferritin-immune complex labeling scattered over the membrane surface. This technique should provide a sensitive and relatively simple method for the ultra structural detection of other immunoglobulin determinants.

Footnotes

¹ This study was supported by United States Public Health Service research Grant AI 09719-01.

This article has been cited by other articles:

				S. Sell and H. W. Sheppard Jr. Rabbit Blood Lymphocytes May Be T Cells with Surface Immunoglobulins Science, November 9, 1973; 182(4112): 586 - 587. [Abstract] [PDF]