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M Alloantibody Elicited by First-Set Renal Allografts: in Vivo and in Vitro Studies¹

From the Department of Experimental Pathology, Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037

Abstract

M alloantibody (allo M) was produced in Lewis (Le) rats after a 5- to 7-day residence of Brown Norway (BN) renal grafts. The isolated, purified and isotopically labeled allo M fixed to spleen, lung, liver and erythrocytes after intravenous injection into BN target rats, but did not fix to kidney unless the renal vein was clamped for 3 min. Allo M fixed promptly in BN kidneys grafted into Le recipients. In such kidneys allo M specifically bound to endothelia of glomerular and intertubular capillaries and of medium-sized arteries. It did not bind in vivo to renal tubular cells but did so in vitro. At least two M antibodies were detected. One reacted with kidney, spleen cells and erythrocytes, the second only with spleen cells. Intravenous infusion of allo M into target BN rats produced extensive pulmonary hemorrhages but the production of these lesions lacked specificity. Allo M elicited by renal graft was strain but not organ specific, i.e., it reacted with transplantation antigens shared by kidney and by other organs and tissues of the immunizing strain. Its role in the rejection process could not be established.

Footnotes

¹ This is Publication No. 550 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. The work was supported by United States Public Health Service Grant AI-07007 and Atomic Energy Commission Contract AT(04-3)-779.

² Supported by a San Diego County Heart Association Fellowship. Present address: University Hospital, Renal Unit, Leiden, The Netherlands.