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The Influence of Calcium, Magnesium and Cyclic Adenosine 3',5'-Monophosphate on the Mixed Lymphocyte Reaction¹

From the Ontario Cancer Treatment and Research Foundation, London Clinic, Departments of Therapeutic Radiology and of Biophysics, University of Western Ontario, London 15, Ontario

Abstract

EDTA inhibits lymphocyte transformation in the mixed lymphocyte culture reaction as it does in PHA containing cultures. The effect is most likely due to binding of Ca⁺⁺ and Mg⁺⁺ required for the initiation and completion of the response since transformation does not occur in cultures deficient in these ions. Exogenous cyclic adenosine 3',5'-monophosphate (cAMP) can potentiate or inhibit the mixed lymphocyte reaction depending on concentration and duration of exposure. The possible interaction of divalent cations and cAMP in lymphocyte transformation is discussed.

Footnotes

¹ This work was supported by the Ontario Cancer Treatment and Research Foundation, London Clinic, London 15, Ontario, Canada.