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The Journal of Immunology, 1972, 108: 913-920.
Copyright © 1972 by The American Association of Immunologists, Inc.
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A Radioactive Antigen-Binding Assay for Neisseria Meningitidis Polysaccharide Antibody

Brenda L. Brandt, Frederic A. Wyle1 and Malcolm S. Artenstein

From the Department of Bacterial Diseases, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D. C. 20012

Abstract

A modification of the Farr technique (see reference 7) was used to develop a highly sensitive and specific radioactive antigen-binding assay for the detection of antipolysaccharide antibody to the groups B and C meningococci. Labeled polysaccharide was extracted from 16-hr culture supernatants of organisms grown in modified Frantz medium containing 14C sodium acetate. The polysaccharide was precipitated by Cetavlon and purified by CaCl2 extraction, ethanol fractionation and Sepharose 4B chromatography.

The assay was shown to be highly reproducible and group specific. An unexpected cross-reaction was demonstrated between certain anti-B antisera with the group C antigen. Complete agreement was found between the group C antigen-binding assay and the group C hemagglutination test in detecting antibody rises in adults who were asymptomatic nasopharyngeal carriers, clinical cases or vaccine recipients. Nineteen paired sera from group B meningitis patients all showed antibody rises in the B antigen-binding assay whereas only 14 showed rises by the B hemagglutination test. Only 3 of 11 group B nasopharyngeal carriers showed an antibody response in the radioactive antigen-binding test. In young children immunized with the group C polysaccharide vaccine, the sensitive group C antigen-binding assay demonstrated antibody rises in 93% of the children, most of whom showed borderline or negative response as measured by the hemagglutination test.

Footnotes

1 Present address: Frederic A. Wyle, M.D., Long Beach VA Hospital, Long Beach, California 90801.






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