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In Vitro Responses of Peripheral Blood Lymphocytes in Experimental Allergic Encephalomyelitis¹

From the Department of Anatomy, Medical Center, University of Kentucky, Lexington, Kentucky 40506

Abstract

Tritiated thymidine incorporation was determined in cultures of peripheral leucocytes taken from rabbits in which experimental allergic encephalomyelitis (EAE) was produced by immunization against either whole brain (WB) or myelin basic protein (BP). There was a significant increase in ³H-thymidine uptake in cultures of lymphocytes of animals with EAE induced with WB when stimulated with WB, but there was no increase with BP added to these cultures. When the lymphocyte donors were rabbits in which EAE was produced by BP, ³H-thymidine incorporation was increased significantly by the addition of either WB or BP to the cultures. Lymphocytes of all donors responded to stimulation in vitro before the onset of symptoms of EAE and failed to respond after the disease progressed. Lymphocytes taken from animals given protective injections of either WB or BP failed to respond to stimulation by these antigens.

Footnotes

¹ This investigation was supported by a grant from the National Multiple Sclerosis Society.