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Local Synthesis of Secretory IgA in Experimental Pyelonephritis^1,2,

From the Department of Internal Medicine, Veterans Administration Hospital, 4500 South Lancaster Road, Dallas, Texas 75216, and the University of Texas Southwestern Medical School at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas 75235

Abstract

Studies were performed to determine if secretory IgA was synthesized by kidney following hematogenous and retrograde urinary tract infection in rabbits. The synthesis of secretory IgA was analyzed by comparing the percentage of radioactivity (¹⁴C) precipitated by an antiserum to secretory piece to that precipitated by an antiserum to colostral IgA. The IgA synthesized by pyelonephritic kidney was precipitated by anti-secretory piece, whereas IgA synthesized by the spleen was not. Newly synthesized IgA from pyelonephritic kidney migrated immunoelectrophoretically as did colostral IgA. These studies confirm that newly synthesized IgA is secretory IgA. Reduction and alkylation of secretory IgA was followed by disc electrophoresis in alkaline urea gel. These studies demonstrated that newly synthesized IgA contained heavy, light and "J" chain. Since secretory IgA was synthesized late in the inflammatory response in hematogenous pyelonephritis and was not synthesized in mild pyelonephritis produced by retrograde route, extensive tubular epithelial destruction appears to be central to the synthesis of secretory IgA in pyelonephritis. No specific cell-binding antibody activity was demonstrated for newly synthesized secretory IgA by pyelonephritic kidney in these studies.

Footnotes

¹ This work was supported by Veterans Administration Hospital Research, and by the United States Health Service Research Grant 5 R01 HD 00851 from the National Institute of Child Health and Human Development.

² Requests for reprints should be addressed to Dr. James W. Smith, General Medical Research, Infectious Disease Section, 4500 South Lancaster Road, Dallas, Texas 75216.

³ Dr. Smith was a recipient of Research Career Development Award 1-K04-A1-05097 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service, during this study.

⁴ Dr. Hand was a recipient of Special Fellowship 1-F3-A1-40, 467 from the National Institute of Allergy and Infectious Diseases, United States Public Health Service. His present address is Veterans Administration Hospital and Emory University School of Medicine, Atlanta, Georgia.