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Stimulation of B-Lymphocytes by Endotoxin

Reactions of Thymus-Deprived Mice and Karyotypic Analysis of Dividing Cells in Mice Bearing T₆T₆ Thymus Grafts¹

From the Department of Microbiology, Yale University School of Medicine, New Haven, Connecticut, 06510

Abstract

Thymus-independent B lymphocytes were shown to play the main role in the transformation response of mouse spleen cultures to endotoxin (LPS). Spleen cells from mice thymectomized at 6 to 8 weeks, lethally irradiated, and reconstituted with syngeneic bone marrow (TxXBM) reacted normally to LPS, while the responses to phytohemagglutinin and ConA were markedly depressed. The reaction to LPS, however, was temporarily depressed in the TxXBM mice and was fully recovered only 4 weeks after treatment. This latent period may be necessary for processing of the reacting cells. Karyotypic analysis of spleen cultures from mouse chimeras with T₆T₆ thymus grafts showed that only 1.2% of the LPS-stimulated cells were of thymus origin, as compared to 29.7% reacting to PHA and 12.2% to sheep red blood cells.

Footnotes

¹ This work was supported by United States Public Health Service Research Grants AI-06112 and AI-06455.

² On leave of absence from the Department of Medical Ecology, Hebrew University-Hadassah Medical School, Jerusalem.

³ Fellow of the Deutsche Forschungsgemeinschaft.

⁴ Supported by United States Public Health Service Training Grant AI-00291.

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