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Electrophoretic and Antigenic Analysis of Mouse and Guinea Pig Anaphylactic Antibodies¹

From the Department of Pathology, New York University Medical Center, New York, New York, and the Laboratory of Immunogenetics, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

Abstract

Mice and guinea pigs were immunized with dinitrophenyl (DNP) protein complexes in low doses combined with aluminium hydroxide gel adjuvant. This scheme led to the production of two different types of antibodies mediating passive cutaneous anaphylaxis (PCA) reactions in each species. Both species made a heat stable IgG₁ antibody capable of giving PCA reactions with a short sensitization period, which was removed by treatment with specific anti-IgG₁ serum, and migrated electrophoretically with the total IgG₁ globulins of each species. The other antibody provoking PCA reactions in each species was detected with a longer sensitization period (2 days in mice and 4 days in guinea pigs). These antibodies did not show identical properties to each other. The mouse antibody was completely heat labile, was not removed by a specific anti-IgG₁ serum, and migrated electrophoretically slightly more slowly than did IgG₁. In distinction, the guinea pig antibody was only marginally affected by heating, was removed by pretreatment with a specific anti-IgG₁, and had a faster electrophoretic mobility than IgG₁ globulin. These results support a previous observation of Parish, that guinea pigs may possess two heat stable IgG₁-type antibodies that can mediate PCA, possibly as well as having a true reaginic antibody.

Footnotes

¹ This work was supported by National Institutes of Health Grants AI-03075-13 and AM-11234-05.

² Department of Pathology, New York University Medical Center, New York, New York 10016.

³ Laboratory of Immunogenetics, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia.