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Phylogeny of Immunocompetent Cells

I. In Vitro Blastogenesis and Mitosis of Toad (Bufo Marinus) Splenic Lymphocytes in Response to Phytohemagglutinin and in Mixed Lymphocyte Cultures¹

From the Division of Immunology of the Department of Microbiology, University of Rochester School of Medicine and Dentistry, Rochester, New York 14642

Abstract

Toad splenic lymphocytes undergo blastogenic conversion in vitro in response to both phytohemagglutinin (PHA) and allogeneic splenocytes. At 26°C, maximum incorporation of tritiated thymidine occurs on day 3 in response to PHA, and on day 7 in a mixed lymphocyte culture (MLC). The response to PHA is concentration-dependent. The maximum response to PHA and to alloantigens varies from toad to toad, and is influenced by the type of serum used to supplement the culture medium. More lymphocytes cultured with xenogeneic serum and PHA undergo mitosis than those cultured with PHA and allogeneic serum. In contrast, allogeneic serum suppresses transformation in the MLC.

Footnotes

¹ This work was supported by Grant AI-08784 from the United States Public Health Service.

² Supported by United States Public Health Service Training Grant 5-R0IGM00592 and by a NDEA Graduate Fellowship 69-02457.

³ To whom correspondence concerning the manuscript should be addressed.