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Studies on Human Secretory Immunoglobulin A

II. Subunit Structure¹

Institute of Dental Research and the Department of Microbiology, School of Dentistry, University of Alabama in Birmingham, Alabama 35233

Abstract

Secretory IgA (S-IgA), in dissociating media, released small portions of secretory component (SC) and light chains but no J chain. After oxidative sulfitolysis, or reduction and alkylation followed by gel filtration in 5 M guanidine-HCl, SC was eluted in the heavy chain fraction, and J chain in the light chain fraction. A considerable portion of SC and J chain was released by oxidative sulfitolysis in the absence of dissociating agents and separated by gel filtration in neutral buffer. Data indicate one SC and one J chain per molecule of 11S S-IgA. The proposed molecular formula is (₂L₂)₂J₁SC₁.

Footnotes

¹ This investigation was supported in part by United States Public Health Service Grants DE 02670 and DE 03169, and by the John A. Hartford Foundation, Inc.

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