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Antibody Affinity and Valence in Viral Neutralization¹

Department of Immunology and Medical Microbiology, College of Medicine, University of Florida, Gainesville, Florida 32601

Abstract

This work shows that viral neutralization appears to be a function not only of antibody concentration but also of affinity, valence, and possibly molecular configuration of antibody. When antibody molecules of similar valence are grouped, neutralization increases with increasing molecular affinity. Conversely, if antibody molecules have comparable affinities, an increase in valence markedly increases neutralization. From these studies it appears that valency is perhaps the greater factor contributing to the neutralizing efficiency of antibody molecules. These findings also seem to indicate that the phage neutralization assay may not be accurate for quantitative antibody comparisons.

Footnotes

¹ This work represents a portion of a thesis submitted by Susan Erma Blank to the Graduate Council of the University of Florida in partial fulfillment of the requirements for the Master of Science degree.

² NDEA Title IV predoctoral fellow.

³ Postdoctoral Research Fellow, Award 1 FO2 AI 44187-01 from the United States Public Health Service. Present address: Department of Microbiology, Tulane University School of Medicine, New Orleans, Louisiana.

⁴ Supported by National Science Foundation Grant GB-8632. Reprint requests should be sent to Dr. Clem.

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