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Release of Histamine and Slow Reacting Substance of Anaphylaxis (SRS-A) by IgE-Anti-IgE Reactions on Monkey Mast Cells¹

Departments of Medicine and Microbiology of the Johns Hopkins University, and the O'Neill Memorial Laboratories of The Good Samaritan Hospital, Baltimore, Maryland

Abstract

Lung cell suspensions were obtained from Macaca irus passively sensitized with E myeloma protein and human IgG. By autoradiography the myeloma protein was detected on mast cells whereas IgG was detected on macrophages and neutrophil granulocytes. Evidence was obtained that almost all histamine in the lung cell suspension was associated with mast cells. Histamine base per monkey mast cell was 3 to 5 pg. The reaction between mast cell-bound IgE and anti-IgE released both histamine and SRS-A, whereas IgG-anti-IgG reaction on macrophages released neither chemical mediator. The release of the chemical mediators by IgE-anti-IgE reaction on mast cells was inhibited by isoproterenol and by theophylline.

Footnotes

¹ This research was supported by Research Grants AI-10060 from the United States Public Health Service and GB-27682 from the National Science Foundation. This paper is publication No. 17 from the O'Neill Laboratories, The Good Samaritan Hospital.