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Migration Inhibitory Effect of Cell-Free Supernatants from Mixed Human Lymphocyte Cultures¹

Medical Department A and the Laboratory for Clinical Immunology, Medical Department TA, Rigshospitalet, University Hospital, Copenhagen, Denmark

Abstract

Cell-free supernatants from mixed human lymphocyte cultures inhibited the migration of non-separated human peripheral leucocytes and purified polymorphonuclear leucocytes. The maximum inhibitory effect was observed in 4-day cultures, but even after 2 days inhibitory effect was demonstrated. The migration inhibitory factor (MIF) from mixed lymphocyte cultures was non-dialyzable, 56°C heat-stable and non-sedimentable at 78,000 x G. MIF was produced also in mixed cultures of lymphocytes pretreated with mitomycin C although the inhibitory effect was diminished in most of these experiments. The migration inhibition was demonstrated with an agarose plate technique which permits examination of only a few hundred microliters of unconcentrated MIF-containing supernatant.

Footnotes

¹ This study was supported by grants from the Danish State Foundation for Science and the Danish Foundation for the Advancement of Medical Science.