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The Effect of Localized Injection of Adjuvant Material on the Draining Lymph Node¹

III. Thymus Dependence

Transplantation Immunology Laboratory, Departments of Medicine and Surgery, Mount Sinai School of Medicine, New York, New York and the Department of Pathology, Yale University School of Medicine, New Haven, Connecticut

Abstract

The adjuvant effects of killed Bordetella pertussis organisms on the immune response to heterologous globulin was studied in normal, "T-cell-depleted" (thymectomized, irradiated, bone-marrow reconstituted) and thymus-marrow chimeric CBA mice.

Incorporation of B. pertussis with alum-precipitated equine globulin (EGG) as antigen potentiated the anti-EGG antibody response in normal mice, but not in T-cell depleted animals.

The draining lymph nodes of normal animals injected in the hind footpad with alum-precipitated bovine globulin (alum-BGG) showed a gradual rise in weight, followed by an increase in DNA synthetic activity as judged by uptake in the node of ¹²⁵I-labeled 5-iodo-2-deoxyuridine (IUDR). Normal animals given pertussis-incorporated antigen showed an accelerated and augmented lymph node weight increase, and a higher peak of DNA synthesis. In T-cell-depleted animals, lymph node weight and IUDR uptake were only slightly increased after alum-BGG, and were not further enhanced by using alum-BGG-pertussis.

Mitoses were more numerous in the draining nodes of CBA/HT6T6-CBA/H thymus-marrow chimeras given alum-BGG-pertussis than in such animals given alum-BGG only. At any given time during the response, however, the ratio of thymus-derived to marrow-derived mitoses was approximately the same in the two groups.

Normal mice given alum-BGG-pertussis showed histologic hyperplasia of the paracortical regions of draining lymph nodes, with increased numbers of small lymphocytes. In T-cell-depleted animals, paracortical hyperplasia occurred without a significant increase in colonizing small lymphocytes.

The data suggest that B. pertussis organisms act to augment the recruitment of recirculating thymus-derived cells to the paracortical regions of draining lymph nodes, and that subsequent cellular proliferation in the node is in part determined by the magnitude of this recruitment.

Footnotes

¹ Aided in part by United States Public Health Service Grants CA-08593 and CA-1282701 National Cancer Institute.

² Scholar of the Leukemia Society of America, Inc.

³ Recipient of United States Public Health Service Career Development Award (CA-10-316) from the National Cancer Institute.