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The Antibody Response of Rabbits to Soluble Mouse H-2 Antigens¹

Transplantation Unit of the General Surgical Services, Massachusetts General Hospital, and the Department of Surgery, Harvard Medical School, Boston, Massachusetts

Abstract

Soluble H-2 transplantation antigens were prepared from H-2^d L1210 leukemia cells by sonication and an (NH₄)₂SO₄ precipitation of this soluble material was used to immunize rabbits. The antisera produced reacted broadly in a cytotoxic test with the spleen and tumor cells of many different inbred mouse strains, but, after absorption, reacted specifically with several H-2^d alloantigens. "K region" antigens were more immunogenic than "D region" antigens, especially the "private" specificity H-2.31. The rabbit anti-mouse sera contained no detectable non-H-2 antibodies nor antibodies reactive with tumor specific antigens and was unable to form precipitin lines in agar gel. The immunosuppressive activity of this serum, tested by skin grafting, was negligible.

Footnotes

¹ Supported by research Grants from the United States Public Health Service, No. AI-06918 and No. AI-06320, and by National Institute of Allergy and Infectious Diseases, National Institutes of Health Contracts No. PH 43-69-90 and No. AM-07055.