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Neutrophilic Leukocytes in Immunologic Reactions: Evidence for the Selective Release of Lysosomal Constituents¹

Division of Clinical Immunology, Department of Medicine, Montreal General Hospital and the McGill University Medical Clinic of the Montreal General Hospital, Montreal 109, Quebec, Canada

Abstract

The release of lysosomal bioactive factors is essential to the role of the neutrophilic leukocyte as a mediator of tissue injury in immunologic inflammatory lesions. The lysosomal release process was studied by exposing rabbit neutrophils to phagocytosable immune complexes, non-phagocytosable immune complexes and antineutrophil antibody and measuring the kinetics of release of a lysosomal marker enzyme ( glucuronidase). All three types of immunologic stimuli produced a rapid, selective release of the lysosomal marker. This release preceded that of a cytoplasmic marker enzyme (lactic dehydrogenase) and was not associated with a general increase in cell membrane permeability as judged by trypan blue dye exclusion. In contrast when neutrophils were exposed to a cytotoxic chemical, N-ethylmaleimide, the opposite pattern was observed. Lactic dehydrogenase was rapidly released while release of glucuronidase was greatly delayed. These studies indicate that there is a non-cytotoxic, non-cytolytic mechanism for the immunologically induced release of neutrophil lysosomal bioactive factors. This process may be similar to the non-cytolytic release mechanisms described for other cells such as platelets and mast cells.

Footnotes

¹ This investigation was supported by a grant from the Medical Research Council of Canada.

² Scholar, Medical Research Council of Canada.

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