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Binding in Vivo of Enhancing Antibodies to Skin Allografts and Specific Allogeneic Tissues¹

Scripps Clinic and Research Foundation, 476 Prospect Street, La Jolla, California 92037

Abstract

Binding of antibodies with enhancing quality to target skin grafts and to allogeneic tissue was studied in vivo. The alloantibodies were ¹²⁵I-labeled enriched IgG fraction (¹²⁵I-EIgG) of Lewis (Le) antisera and were paired with ¹³¹I-labeled IgG (¹³¹I-ClgG) of control Le sera. ¹²⁵I-EIgG was specifically bound to target skin grafts within 2 days after grafting and was blocked from localizing in skin grafts by unlabeled enhancing antisera given at the time of grafting ("afferent" blockade). Alloantigenic sites were blocked for several days, after which time skin grafts bound ¹²⁵I-EigG for up to 40 days. Blocking in vitro of alloantigenic sites was demonstrated and quantitated. Enhancing antibodies generated by long-term enhanced skin grafts on Le hosts (auto-enhancement) were not detected in the circulation. ¹²⁵I-EIgG disappeared from the circulation of allogeneic rats and was specifically localized in spleen, lung and liver, but not in kidney. ¹²⁵I-EIgG persisted in the circulation of syngeneic hosts and was not specifically localized in their organs.

Footnotes

¹ This is Publication No. 548 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California. The work was supported by United States Public Health Service Grant AI-07007 and Atomic Energy Commission Contract AT(04-3)-779.

² Supported by United States Public Health Service Training Grant 5TIGM683-09.

³ Supported by Deutsche Forschungsgeneinschaft, 5320 Bad Godesberg, Kennedy-allee 40, Forschungsstipendium PE 151/2.