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Immunoglobulin M: Identification of an Unusual Fragment Following High Temperature Trypsin Proteolysis¹

Department of Medicine, School of Medicine State University of New York at Buffalo, Buffalo, New York 14814

Abstract

A fragment (Int-L) of m.w. 20,000 containing 25% carbohydrate has been identified in (Fc)_5µ preparations obtained from high temperature trypsin digests of human type IgM molecules. This fragment consists of two disulfide linked components, one related to light chains and the second a portion of µ chain which presumably contains the carbohydrate. The intact fragment is univalent with respect to most anti- antisera which precipitate it only following reduction of disulfide bonds.

The Int-L has not been precisely localized in the IgM molecule. Some evidence indicates it may arise from outside the Fab fragment raising the possibility that it represents a third L chain per IgM subunit.

Footnotes

¹ This work was supported in part by National Institutes of Health Grant No. 5 RO1 AM 10419 from the United States Public Health Service, and Henry and Bertha Buswell Research Fellowship.

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