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In Vitro Cooperation of Cells of Bone Marrow and Thymus Origins in the Generation of Antibody-Forming Cells¹

Scripps Clinic and Research Foundation and the Department of Biology, University of California, San Diego, La Jolla, California 92037

Abstract

B cells and T cells cooperate in the in vitro generation of hemolytic plaque-forming cells to sheep, horse and burro erythrocytes. The B cells are obtained from the spleens of lethally irradiated mice injected with bone marrow cells. The T cells are obtained from the spleens of lethally irradiated mice injected with thymus cells and heterologous erythrocytes. Both cell populations are obtained after 7 days residence in the irradiated hosts. Neither cell population can respond when cultured alone. The in vitro function of B cells, but not T cells, is inactivated by exposure to 2000 R irradiation. The T cells function best in in vitro response to the erythrocyte antigens with which they were injected into the irradiated hosts. Responses were also generated to other foreign erythrocytes and to the trinitrophenyl hapten coupled to erythrocyte carriers.

Footnotes

¹ This is Publication No. 539 from the Department of Experimental Pathology, Scripps Clinic and Research Foundation, La Jolla, California 92037. This work was supported by grants from United States Public Health Service, Training Grant GM00683, AI-08795-02, and American Cancer Society, E-395D and Cancer Research Coordinating Committee, University of California.

² Supported by United States Public Health Service Training Grant GM00683. Present address: Department of Genetics, School of Medicine, University of Hawaii, Honolulu, Hawaii 96822.

³ The Department of Biology, University of California, San Diego, La Jolla, California 92037.