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Genetically Determined Immune Responses: In Vitro Studies¹

From the Stanford Research Institute, Menlo Park, California 94025

Abstract

Blood leukocytes, spleen, bone marrow, and fetal liver cells from strains of mice that respond well (HR) or poorly (LR) to the synthetic polypeptide (T,G)-A—L were challenged in vitro with this antigen. At the peak of the response, there were 2 to 100 times more HR than LR blood, spleen, and marrow cells proliferating; however, there was no detectable difference when stem cells from HR and LR embryos were stimulated. An antiserum to the antigen greatly reduced the responses to (T,G)-A—L of HR and LR blood, spleen, and marrow cells; this antiserum had no effect on the response by cells from fetal liver. Spleen-incubated HR thymus cells were more effective than similarly treated cells from LR mice in increasing the proliferation of fetal lymphoid cells challenged with (T,G)-A—L. The results suggest that LR strains respond poorly to (T,G)-A—L because of an antigen-induced cell membrane change that affects primarily the function of the thymus-passaged cell; this change impedes contact between thymus-passaged cells and the specific antibody-producing precursors with the result that proliferation and/or recruitment of the latter is impaired.

Footnotes

¹ Supported by the Office of Naval Research Contract N00014-70-C-0068.

² Present address: Dental Research Center, Chapel Hill, North Carolina 27514.