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Electrokinetic Behavior of Intermediate Cells in Immune Hemolysis¹

From the National Cancer Center Research Institute, Tokyo, Japan, and the Aichi Cancer Center Research Institute, Nagoya, Japan

Abstract

Electrophoretic mobility of intermediate cells was increased with the reaction of C2 and further increased with the addition of C3. Less than 18 molecules of C2 per cell produced detectable change in electrophoretic mobility. The increase in mobility by the formation of C2 sites diminished with the decay of C2 sites. C1 is required to maintain the high mobility of EAC142, EAC1423 or EAC143. No change in the mobility of intermediate cells was observed by the reaction of C5, C6, C7 or C8. After the reaction of C9, the electrophoretic mobility of E* decreased significantly before rupture of the cell.

Footnotes

¹ Part of this work was presented at the Seventh Complement Symposium of Japan (Tokyo, June 1970) and at the Fifth International Symposium on the Biological Activities of Complement of the Canadian Society of Immunology (Guelph, August 1970).

² National Cancer Center Research Institute, Tokyo, Japan.

³ Aichi Cancer Center Research Institute, Nagoya, Japan.