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The Immunosuppressive Effects of Rauscher Leukemia Virus (RLV) upon Spleen Cells Cultured in Cell-Impermeable Diffusion Chambers¹

III. Inhibition of RLV-Induced Cell Pathways by Antigenic Stimulation with Hemocyanin

From the Laboratories of Virology and Immunology, St. Jude Children's Research Hospital and the University of Tennessee Medical Units, Memphis, Tennessee 38101

Abstract

In mouse spleen cell cultures, keyhole limpet hemocyanin (KLH) inhibited the leukemogenic process induced by Rauscher leukemia virus (RLV). Spleen cells obtained from normal or RLV-infected BALB/C mice were cultured with or without KLH in cell-impermeable diffusion chambers that were implanted in isogeneic hosts. The effects of RLV and KLH upon these cells were determined by quantitative analysis of cell morphology and by measurement of in vitro immunoglobulin synthesis. Increases in blast, myeloid, erythroid and monocytoid cells were found in RLV-infected cultures that were not restimulated with KLH. In RLV-infected cultures that were restimulated with KLH, numbers of blasts and hematopoietic cells were within normal range, but there was a marked increase of plasma cells and of in vitro immunoglobulin synthesis. It is postulated that antigenic stimulation by KLH of RLV-infected cultures shifts the differentiation pathways of a pluripotent stem cell from a blast-hematopoietic cell line into a lymphoid-plasma cell line.

The finding that antigenic stimulation of infected cell cultures alters the cellular pathway induced by a leukemogenic virus may provide a new approach to the investigation of regulatory mechanisms and control of cell proliferation and differentiation during leukemogenesis.

Footnotes

¹ This study was supported by United States Public Health Service Grant CA-08480 and by ALSAC.