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Response of Peripheral Blood Lymphocytes from Normal, Hypogammaglobulinemic and Chronic Lymphocytic Leukemic Patients¹

From the Division of Immunologic Research, Milwaukee Blood Center, Milwaukee, Wisconsin and Department of Pediatrics, University of Minnesota, Minneapolis, Minnesota

Abstract

Staphylococcal protein A (SPA) derived from Cowan I strain of Staphylococcus aureus was cultured in vitro with peripheral blood leucocytes of normal donors and of patients with various diseases. Cells from the majority of normal donors proliferate in the presence of SPA. Maximum tritiated thymidine incorporation occurs with 25 to 50 µg SPA per culture and when cultures are incubated 7 to 9 days. Removal of glass adherent cells prior to culture markedly reduces the proliferative response to SPA. Cells of three patients with hypogammaglobulinemia responded vigorously to SPA. These findings suggest that the response to SPA is antigen-specific, rather than nonselective, and that responding lymphocytes are thymus-derived, T-cells. The in vitro model described may provide a tool to study the role of cellular immunity in defense against staphylococci

Footnotes

¹ Supported by grants from the Milwaukee Blood Center, Inc., the Minnesota Division of the American Cancer Society, United States Public Health Service AI 06931 and conducted under the sponsorship of the Commission on Streptococcal and Staphylococcal Diseases, Armed Forces Epidemiological Board.

² Please address all correspondence to Dr. Glenn E. Rodey, Director, Immunologic Research, Milwaukee Blood Center, Inc., 763 North 18th Street, Milwaukee, Wisconsin 53233.

³ Fellow in Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455.

⁴ Professor of Pediatrics, University of Minnesota, Minneapolis, Minnesota 55455.