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The Use of Polyacrylamide Gel as an Immunoadsorbent for Anti-2, 4-Dinitrophenyl Antibodies¹

Institute for Biochemistry, University of Lausanne, Switzerland and the Department of Microbiology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140

Abstract

A simple, effective method for preparing an immunoadsorbent specific for anti-DNP antibody is described. The immunoadsorbent was made with a highly cross-linked polyacrylamide gel containing dinitrophenylated bovine serum albumin (DNP₃₀BSA) as the antigen. The immunoadsorbent permitted the adsorption and subsequent elution of a mouse myeloma IgA (MOPC-315) with anti-DNP activity. The Fc and F(ab) fragments from MOPC-315 could also be separated effectively on this column. In addition, a non-precipitating mouse IgG anti-DNP-hemocyanin antibody was also isolated using the same immunoadsorbent.

Footnotes

¹ This work was supported by the Swiss National Foundation Grant 3361/70 to H. J. and by an American Cancer Society Grant T-488A to H. F. H.

² Career Development awardee (K3-CA-19,409).