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The Dna Synthetic Response of Adoptively Transferred Thymocytes in the Spleens of Lethally Irradiated Mice

I. Effects of Varying Antigen and Thymocyte Doses¹

Department of Pathology, Yale University School of Medicine, New Haven, Connecticut 06510

Abstract

The DNA synthetic response of adoptively transferred thymocytes was measured in the spleens of lethally irradiated syngeneic mice by determining the uptake of ¹²⁵I-labeled 5-iodo-2-deoxyuridine. Sheep red blood cells (SRBC) were used as a source of antigenic stimulation and the effects of varying both the antigen (100-fold range) dose and the thymocyte (50-fold range) dose were studied. The shape of the thymocyte response curve was similar to that of thymus-derived lymphocytes responding in radiation chimeras. At all thymocyte doses studied the largest antigen dose (0.2 ml of packed SRBC) produced the best response. With this antigen dose, no significant variation in peak response was seen over a 10-fold thymocyte dose range although the peak occurred earlier with the highest thymocyte dose. These results suggest that this technique is useful for studying the response of thymocytes to antigen and that the response itself is a complex one.

Footnotes

¹ This work was supported by Grant CA-08593 from the National Cancer Institute, Bethesda, Md., and a subgrant from the ACS, IN-31.

² Richard K. Gershon is the recipient of a Career Development Award (CA-10316) from the National Cancer Institute, Bethesda, Md.