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Studies on Rabbit Lymphocytes in Vitro

XIV. Fractionation of Rabbit Peripheral Blood Lymphocytes by Antibody-Coated Polyacrylamide Beads

From the Department of Pathology, University of California, San Diego, School of Medicine, La Jolla, California 92037

Abstract

Polyacrylamide beads 400 µ to 800 µ in diameter were covalently coated with antibody globulin and the coated beads were used to fractionate lymphocyte populations. Beads coated with anti-lymphocyte, anti-immunoglobulin and anti-allotypic globulins bound rabbit peripheral blood lymphocytes. Adherent cells were eluted from coated beads after non-adherent cells had been washed off. The eluted population of lymphocytes adherent to anti-immunoglobulin- or anti-allotypic globulincoated beads was enriched in cells that contain detectable immunoglobulin antigens. Although only partial separation was obtained, it was concluded that this method might be applied with more satisfactory results to cell mixtures of other types.