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The Isotopic Antiglobulin Assay: Application to the Study of Hybrid Cell Membranes

From the Department of Biology, University of Utah, Salt Lake City, Utah 84112

Abstract

An isotopic antiglobulin assay was used to study the cell surface antigens of a hamster-mouse hybrid cell. Antisera were developed against the nuclei and whole cells of the parents and the hybrid. Whereas antisera to hamster and mouse whole cells cross-reacted extensively, antisera to nuclei were species-specific. The antinuclear sera were used to demonstrate the presence of hamster-specific and mousespecific cell surface antigens on the hybrid cell. The proportion of these antigens on the hybrid cell surface closely mirrored the number of chromosomes contributed by each parent. Antisera to hamster and mouse whole cells gave the same antiglobulin binding on the hybrid. Antiglobulin saturation of the hybrid cell surface by antiserum to either parent implied intimate mixing of hamster and mouse cell surface antigens. Further evidence for molecular intermixing was obtained by demonstrating that the binding of hamster-specific globulin to the hybrid cell surface prevented binding of mouse-specific globulin.