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Detection of Anti-Phage Antibody by Passive Hemagglutination

II. Early Antibodies Produced in Vivo or in Vitro¹

From the Department of Immunology, The Public Health Research Institute of The City of New York, Inc., New York, New York 10016

Abstract

A hemagglutination test capable of detecting 2.5 x 10^-5 µg of antibody nitrogen was applied to the measurement of anti-T2 phage activity during early phases of the antibody response induced in vivo or in vitro. Using this test serum antibody was detected within 24 hr of immunization and rose rapidly, whereas conventional neutralization tests indicated a slow increase in activity. Neutralization tests in the presence of complement indicated a rise in activity with time that paralleled the increase in hemagglutination activity. Antibody induced in vitro was detected by the conventional neutralization test only with difficulty but was readily demonstrable by the hemagglutination test.

Footnotes

¹ This work was supported by Grant AI-06899 from the National Institute of Allergy and Infectious Diseases and is presently supported by Grant GB-29018X from the National Science Foundation.

² Supported by a Postdoctoral Fellowship (AI-25,603) and a Training Grant (T01-AI-00408) from the National Institute of Allergy and Infectious Diseases.