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Increased Complement Efficiency through Control of Ionic Strength and Temperature¹

From the Department of Microbiology, University of California, San Francisco, San Francisco, California 94122

Abstract

When complement (C) was in relative excess and IgG antibody (Ab) was limited, a great increase in hemolytic efficiency was obtained by incubating sensitized red cells with C for 1 hr at 0°C, µ = 0.037, followed by 1 hr at 37°C, µ = 0.094. When IgG Ab was in relative excess and C was limited, this effect was much smaller. C1 binding affinity seemed to be the most important factor in determining the hemolytic efficiency. With IgM Ab, a smaller but still significant (65%) increase in hemolytic efficiency was obtained by a one-step incubation at 37°C, µ = 0.094. When direct lysis by whole C was compared with C1 fixation and transfer, up to 20% of the IgG sites capable of fixing C1 could be converted to S* under optimal conditions of temperature and ionic strength.

Footnotes

¹ This work was supported in part by American Cancer Society Grant E-529.