HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Liakopoulou, A. Articles by Perelmutter, L. Search for Related Content PubMed Articles by Liakopoulou, A. Articles by Perelmutter, L.

Antigenic Relationship Between Human IgE Immunoglobulin and Rat Homocytotropic Antibody

Pharmacology Division, Food and Drug Directorate, Department of National Health and Welfare, Ottawa, Ontario, Canada

Abstract

Homocytotropic antibody was produced in albino Wistar rats following immunization with bovine serum albumin (BSA) and killed Bordetella pertussis. Peritoneal mast cells from the sensitized animals obtained between the 13th and 15th days after primary injection underwent characteristic antigen-induced morphologic changes when they were brought into contact with BSA, anti-human IgE (anti-IgE), or antihuman Bence Jones (anti-BJ) sera. Similarly, lung preparations from the sensitized rats released histamine in vitro upon challenge with the same antigens. Neither the cells nor the lung tissue responded to anti-human IgG (anti-IgG) serum. In addition, cells and lung preparations from normal animals were not altered morphologically nor released histamine on incubation with either BSA or anti-human immunoglobulin sera. The percentage of morphologically altered cells and the amount of histamine released from the sliced lung suspensions by anti-IgE or anti-BJ () sera were comparable to those induced by specific antigen toward which the animals were sensitized. These results indicated that rat homocytotropic antibody has antigenic determinants in common with the heavy chain of human IgE immungolobulin and the Bence Jones protein.